Óragének (és a melatonin)

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1 Óragének (és a melatonin)
Rékási Zoltán PTE ÁOK Anatómiai Intézet

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4 birds mammals

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9 Melatonin elválasztás explantált patkány tobozmirigyből
normál in vitro megvilágítási ciklusban

10 Melatonin elválasztás explantált csirke tobozmirigyből
normál in vitro megvilágítási ciklusban

11 MELATONIN LIGHT NORADRENERGIC and PEPTIDERGIC PATHWAYS
Adrenergic and VPAC-RECEPTORS SIGNAL TRANSDUCTION CH 2 NH HO N H SEROTONIN N-acetyltransferase (AA-NAT) AcCoA CH 2 HO N H NH CH 3 O C N-ACETYLSEROTONIN hydroxyindol-o-methyltransferase (HIOMT) SAdM CH 3 O N H CH 2 MELATONIN SYNTHESIS NH C CH 3 O MELATONIN MELATONIN RELEASE

12 MELATONIN ? ? ? LIGHT PHOTORECEPTORS SIGNAL TRANSDUCTION
CH 2 NH HO N H CLOCK (PACEMAKER) Clock-genes SEROTONIN ? N-acetyltransferase (AA-NAT) AcCoA CH 2 HO N H NH CH 3 O C N-ACETYLSEROTONIN hydroxyindol-o-methyltransferase (HIOMT) SAdM CH 3 O N H CH 2 MELATONIN SYNTHESIS NH C CH 3 O MELATONIN MELATONIN RELEASE

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14 Four-gene core transcription-translation loop
period timeless TIM PER Clock CLOCK BMAL1 Bmal1 Activation Inhibition DELAY gene expression time

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16 Clock-genes in Drosophila and mammals
PERIOD1 PERIOD PERIOD2 PERIOD3 NLS bHLH/PAS NLS NLS NLS TIMELESS CLOCK CYCLE BMAL1 Doubletime casein kinase I epsilon Cryptochrome1 Cryptochrome Cryptochrome2

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19 Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken pineal gland

20 Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken retina

21 Expression of retinal and pineal cAANAT mRNA in chicken housed in constant darkness for 1 day or 1 week retina pineal

22 Expression of retinal and pineal cBmal1 mRNA in chicken housed in constant darkness for 1 day or 1 week retina pineal

23 Expression of retinal and pineal cAANAT mRNA in chicken housed in constant light for 1 day or 1 week

24 Expression of retinal and pineal cBmal1 mRNA in chicken housed in constant light for 1 day or 1 week

25 Model of circadian clock-work within an individual retinal or pineal photoreceptor cell
Resetting the clock by light Clock Bmal1 cCry2 cCry1 cPer2 cPer3 cAANAT Output/melatonin

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